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plasmids pcmv flag  (OriGene)


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    Structured Review

    OriGene plasmids pcmv flag
    Plasmids Pcmv Flag, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+pcmv+flag/pCMV6-Entry+Mammalian+Expression+Vector/pm41904194-79-0-3
    Average 96 stars, based on 1672 article reviews
    plasmids pcmv flag - by Bioz Stars, 2026-09
    96/100 stars

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    Construct:

    Article Title: A20 enhances the migration and metastasis of gastric cancer cells by promoting occludin degradation
    Article Snippet: .. Plasmids pCMV-flag ( PS100001 , Origene), pCMV-A20-Myc-flag (RC221337, Origene), occludin cDNA ORF Clone, Human, C-GFPSpark® tag (HG15134-ACG, SinoBiological), and RhoA cDNA ORF Clone, Human, C-OFPSpark® tag (HG12110-ACR, SinoBiological) were constructed and then were used to transfected AGS cells by Lipofectamin 3000. .. Cells were maintained in the continuous presence of G418 (500 μg/mL, A1720, Sigma-Aldrich) or Hygromycin B (200 μg/mL, 31282-04-9, Sigma-Aldrich).

    Article Title: A20 enhances the migration and metastasis of gastric cancer cells by promoting occludin degradation.
    Article Snippet: .. Plasmids pCMV-flag (PS100001, Origene), pCMV-A20-Myc-flag (RC221337, Origene), occludin cDNA ORF Clone, Human, C-GFPSpark® tag (HG15134-ACG, SinoBiological), and RhoA cDNA ORF Clone, Human, C-OFPSpark® tag (HG12110-ACR, SinoBiological) were constructed and then were used to transfected AGS cells by lipofectamin 3000. .. Cells were maintained in the continuous presence of G418 (500 g/mL, A1720, SigmaAldrich) or Hygromycin B (200 g/mL, 31282-04-9, Sigma-Aldrich).

    Transfection:

    Article Title: A20 enhances the migration and metastasis of gastric cancer cells by promoting occludin degradation
    Article Snippet: .. Plasmids pCMV-flag ( PS100001 , Origene), pCMV-A20-Myc-flag (RC221337, Origene), occludin cDNA ORF Clone, Human, C-GFPSpark® tag (HG15134-ACG, SinoBiological), and RhoA cDNA ORF Clone, Human, C-OFPSpark® tag (HG12110-ACR, SinoBiological) were constructed and then were used to transfected AGS cells by Lipofectamin 3000. .. Cells were maintained in the continuous presence of G418 (500 μg/mL, A1720, Sigma-Aldrich) or Hygromycin B (200 μg/mL, 31282-04-9, Sigma-Aldrich).

    Article Title: A20 enhances the migration and metastasis of gastric cancer cells by promoting occludin degradation.
    Article Snippet: .. Plasmids pCMV-flag (PS100001, Origene), pCMV-A20-Myc-flag (RC221337, Origene), occludin cDNA ORF Clone, Human, C-GFPSpark® tag (HG15134-ACG, SinoBiological), and RhoA cDNA ORF Clone, Human, C-OFPSpark® tag (HG12110-ACR, SinoBiological) were constructed and then were used to transfected AGS cells by lipofectamin 3000. .. Cells were maintained in the continuous presence of G418 (500 g/mL, A1720, SigmaAldrich) or Hygromycin B (200 g/mL, 31282-04-9, Sigma-Aldrich).



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    CSPG4 is upregulated by TcdB independent of YAP . A , immunoblots from protein lysates acquired from pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. B , heatmaps depicting Z-score values for YAP regulated genes generated from RNA-seq analysis of pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. C , heatmaps showing Z-score values for MTF1 regulated genes generated from RNA-seq analysis of pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. D , immunoblots from protein lysates taken from HeLa cells transfected for 48 h with control plasmid (pCMV-GFP) or a plasmid for expressing YAP S5A (pCMV-flag YAP2 5SA). For the final 24 h, the transfected cells were exposed to 1 ng/ml of TcdB.

    Journal: The Journal of Biological Chemistry

    Article Title: Clostridioides difficile TcdB induces expression of its receptor (CSPG4) through a noncanonical Hippo signaling mechanism

    doi: 10.1016/j.jbc.2026.111137

    Figure Lengend Snippet: CSPG4 is upregulated by TcdB independent of YAP . A , immunoblots from protein lysates acquired from pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. B , heatmaps depicting Z-score values for YAP regulated genes generated from RNA-seq analysis of pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. C , heatmaps showing Z-score values for MTF1 regulated genes generated from RNA-seq analysis of pericytes exposed for 24 h to 1 ng/ml of TcdB and/or 10 μM XMU-MP-1. D , immunoblots from protein lysates taken from HeLa cells transfected for 48 h with control plasmid (pCMV-GFP) or a plasmid for expressing YAP S5A (pCMV-flag YAP2 5SA). For the final 24 h, the transfected cells were exposed to 1 ng/ml of TcdB.

    Article Snippet: HeLa cells were transfected with the plasmid pCMV-flag YAP2 5SA, which was a gift from Kunliang Guan (Addgene plasmid # 27371; http://n2t.net/addgene:27371 ) ( ).

    Techniques: Western Blot, Generated, RNA Sequencing, Transfection, Control, Plasmid Preparation, Expressing